Reagent- Indole is detected by addition of Kovac’s reagent. IND: Indole Test-production of indole from tryptophan by the enzyme tryptophanase.TDA (Tryptophan deaminase): detection of the enzyme tryptophan deaminase: Reagent- Ferric Chloride.CIT: utilization of citrate as only carbon source.ODC: decarboxylation of the amino acid ornithine by ornithine decarboxylase.LDC: decarboxylation of the amino acid lysine by lysine decarboxylase.ADH: decarboxylation of the amino acid arginine by arginine dihydrolase.ONPG: test for β-galactosidase enzyme by hydrolysis of the substrate o-nitrophenyl-b-D-galactopyranoside.The test kit enables the following tests: All positive and negative test results are compiled to obtain a profile number, which is then compared with profile numbers in a commercial codebook (or online) to determine the identification of the bacterial species. During incubation, metabolism produces color changes that are either spontaneous or revealed by the addition of reagents. They usually detect enzymatic activity, mostly related to fermentation of carbohydrate or catabolism of proteins or amino acids by the inoculated organisms.Ī bacterial suspension is used to rehydrate each of the wells and the strips are incubated. In the API 20E, the plastic strip holds twenty mini-test chambers containing dehydrated media having chemically-defined compositions for each test. The API range provides a standardized, miniaturized version of existing identification techniques, which up until now were complicated to perform and difficult to read. To identify and differentiate members of family Enterobacteriaceae.
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